0.25% Trypsin-EDTA Solution 1X

0.25% Trypsin-EDTA Solution (1X), contains 0.25% gamma irradiated porcine trypsin (1:250) and 0.9 mM EDTA·4Na in HBSS without Ca2+ or Mg2+ and with phenol red.

Catalog Number: TPS-1001
Lot Number: GMP24BS200

Product Details

In adherent cell culture, trypsin or trypsin-EDTA (ethlyenediaminetetraacetic acid) is used for the detachment of cells from a culture dish or flask, and degradation of cell-cell adhesion. Trypsin, a proteolytic enzyme, can cleave collagen that is pre-coated on culture surfaces for cell adhesion.
EDTA, a chelating agent, can bind with cations such as Ca 2+  and Mg 2+  which are involved in cell adhesion. Subculture usually requires chelation of cations and proteolytic reactions, but the severity of the treatment (high concentration or time length) will reduce the viability and the rate of cell growth because of the over-degradation of cell surface proteins and deficiency of cations essential for cells. A protocol should be selected with the least severity possible while ensuring it is compatible with the generation of a single cell suspension of high viability.

TPS-1001 contains 0.25% (w/v) gamma irradiated porcine trypsin and 0.9 mM EDTA·4Na . 2H 2 O in Hanks’ balanced salt solution (HBSS). TPS-1001 is sterile filtered, tested for porcine parvovirus, and is mycoplasma and cell culture tested. The concentration of Trypsin-EDTA is strongly influenced by cell type, culture type, and characteristics of cell growth. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.

Storage/Stability

Trypsin-EDTA solutions should be kept at ≤ -10°C in the dark for long term storage, but can be stored at 2-8°C for one week.  Deterioration of the solution may be recognized by:

• Precipitate or particulate matter throughout the solution
• Cloudy appearance
•  Color change
• pH change