Catalog Number: DME-9011-P

Dulbecco′s Modified Eagle′s Medium Premium (DMEM-Premium) contains L-Alanyl-L- Glutamine, which supplies a longer shelf life. DMEM-Premium significantly improves cell viability and growth.

Strengths

Higher PH Capacity

Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:

Possess a longer shelf life.
More importantly, minimizes toxic ammonia build-up.
Significantly improves cell viability and growth.
Remains stable across a wide range of temperatures.
Improves the culture of primary cells.

Mechanism:

While L-glutamine is an essential amino acid, it tends to break down over time, leading to the formation of harmful byproducts such as ammonia and pyrrolidine carboxylic acid. One strategy to reduce its degradation in culture media is by slowly supplementing it throughout the culture period. However, consistently monitoring and maintaining optimal levels can be labor-intensive and impractical.

A more efficient solution is to use L-alanyl-L-glutamine, which offers significantly greater stability in aqueous environments. Unlike free L-glutamine, it resists degradation and releases aminopeptidases at a controlled rate. This results in a sustained supply of L-glutamine and L- alanine, which are then utilized by cells for protein synthesis and energy metabolism via the TCA cycle.

Dulbecco′s Modified Eagle′s Medium-Premium (DMEM-Premium) contains:

– 5 mM sodium pyruvate
– High glucose (4.5g/L)
– Non-Essential Amino Acids
– 3.7 g/L Sodium Bicarbonate
–  Phenol Red

DMEM-Premium contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).