Dulbecco’s Modified Eagle’s Medium Regular (DMEM-Regular)

Dulbecco’s Modified Eagle’s Medium Regular (DMEM-Regular)

Price range: $20.00 through $181.00

Catalog Number: DME-9010

Dulbecco’s Modified Eagle’s Medium (DMEM) Regular is an enhanced version of Basal Medium Eagle (BME). It is currently among the most commonly utilized cell culture media worldwide.

 

 

 

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Description

While the original formulation of Dulbecco’s Modified Eagle’s Medium (DMEM) included 1000 mg/L of glucose, various glucose concentrations are now commonly used. For instance, GMP Bioscience’s DMEM contains 4.5 g/L of glucose.

In addition, DMEM is enriched with approximately four times the amount of amino acids and vitamins compared to the initial formula. This medium is particularly suitable for culturing primary cells derived from mice and chickens. It is also well-suited for supporting the growth of a wide range of both normal and transformed cell lines.

Each DMEM variant differs in its inclusion of components such as L-glutamine and sodium pyruvate. Notably, the formulation features enhanced buffering capacity due to its 3.7 g/L sodium bicarbonate (NaHCO₃) content, and it requires supplementation with fetal bovine serum (FBS) for optimal cell growth.

Furthermore, DMEM contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).

Dulbecco’s Modified Eagle’s Medium Regular contains

  •  High glucose (4.5g/L)
  •  Non-essential amino acids Sodium pyruvate (0.11 g/L)
  •  Phenol red (0.0015g/L)
  •  L-glutamine (0.585 g/L)
Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Shipping Condition  Room temperature

Additional information

Pack Size

1 × 500 mL, 6 × 500 mL, 1 × 1000 mL
Condition

DMEM – Standard Formulation, DMEM – Standard Formulation (ATCC-based), DMEM – Without L-Glutamine, DMEM – Without Phenol Red, DMEM – Without Sodium Bicarbonate, DMEM – With Sodium Pyruvate, DMEM – Without Glucose
DMEM – With Lipoic Acid, DMEM – Without L-Glutamine & Without Phenol Red, DMEM – Without L-Glutamine & Without Sodium Bicarbonate, DMEM – Without L-Glutamine, Phenol Red & Sodium Bicarbonate, DMEM – Low Glucose (1.5 g/L), DMEM – High Glucose (4.5 g/L), DMEM – Low Sodium Bicarbonate (1.5 g/L), DMEM – 15 mM HEPES (3.6 g/L), DMEM – 25 mM HEPES (5.9 g/L), DMEM – 15 mM HEPES + Low Sodium Bicarbonate (1.5 g/L), DMEM – 25 mM HEPES + Low Sodium Bicarbonate (1.5 g/L), DMEM – Without Phenol Red + 15 mM HEPES, DMEM – Without Phenol Red + 25 mM HEPES, DMEM – Without Phenol Red + Low Sodium Bicarbonate, DMEM – Without Phenol Red + 15 mM HEPES + Low Sodium Bicarbonate

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