Description
Advantages of Dulbecco’s Modified Eagle’s Medium Advance (DMEM Advance) with L‑alanyl‑L‑glutamine
Compared with regular DMEM, GMP Bioscience Dulbecco’s Modified Eagle’s Medium Advance (DMEM Advance) with L‑alanyl‑L‑glutamine offers:
- Higher pH capacity for more stable culture conditions
- 4 mM L‑alanyl‑L‑glutamine (0.869 g/L) instead of L‑glutamine
- Longer shelf life of the medium due to improved glutamine stability
- Minimized toxic ammonia build‑up from glutamine degradation
- Improved cell viability and growth in primary and continuous cell lines
- Stability across a wide range of temperatures
- Enhanced performance for primary cell culture, including sensitive mammalian cells
DMEM Advance with L Alanyl L Glutamine is ideal when you need consistent, high‑quality performance in demanding cell culture workflows.
Why L‑Alanyl‑L‑Glutamine Instead of L‑Glutamine? (Mechanism)
While L‑glutamine is an essential amino acid for mammalian cells, it is chemically unstable in aqueous solution. Over time, L‑glutamine degrades and generates toxic ammonia and pyrrolidone carboxylic acid, which can:
- Stress or damage cells which is proven to be far less in Dulbecco’s Modified Eagles Medium Advance
- Reduce viability and growth
- Compromise experimental reproducibility
One way to address this is to frequently add fresh L‑glutamine to the medium, but this is labor‑intensive and difficult to control precisely which has the same impact.
Dulbecco’s Modified Eagle’s Medium Advance (DMEM Advance) with L‑alanyl‑L‑glutamine solves this problem by using L‑alanyl‑L‑glutamine, a dipeptide form that is:
- Much more stable in aqueous solution
- Resistant to rapid degradation, limiting ammonia formation
- Slowly cleaved by aminopeptidases, gradually releasing L‑glutamine and L‑alanine
- Providing a sustained source of glutamine for protein synthesis and metabolism (e.g., TCA cycle)
The result is healthier cells, less toxicity, and more reproducible results over longer culture periods.
In addition to our DMEM‑Advance formulations, GMP Bioscience also manufactures GMP Bioscience GluaSup, a standalone L‑alanyl‑L‑glutamine supplement designed to prevent L‑glutamine degradation in other media.
DMEM‑Advance Composition and Features
GMP Bioscience Dulbecco’s Modified Eagle’s Medium‑Advance (DMEM‑Advance) contains:
- 4 mM L‑alanyl‑L‑glutamine (0.869 g/L) – stable glutamine source
- 5 mM sodium pyruvate – additional energy substrate
- High glucose (4.5 g/L) – supports metabolically active, fast‑growing cells
- Non‑essential amino acids (NEAA) – reduce biosynthetic burden on cells
- 3.7 g/L sodium bicarbonate – enhanced buffering capacity
- Phenol red – visual pH indicator
GMP Bioscience manufactures several DMEM‑Advance variants based on different sodium bicarbonate and HEPES levels to fine‑tune pH capacity and buffering for your specific incubator CO₂ conditions and experimental requirements.
Serum Supplementation
Like classical DMEM, DMEM‑Advance contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS) or a suitable serum‑free or defined supplement appropriate for your cell type.
Applications of DMEM‑Advance
Dulbecco’s Modified Eagle’s Medium‑Advance (DMEM‑Advance) is recommended for:
- Primary mammalian cell cultures
- Sensitive or fastidious cell lines requiring stable culture conditions
- Long‑term cultures where ammonia build‑up is a concern
- High‑density cultures and scale‑up experiments
- Applications where standard DMEM does not provide sufficient stability or performance, Modified Eagles Medium Advance DMEM works far better and more efficient.
- Lastly based on our decades of experience, Dulbecco’s Modified Eagle’s Medium Advance, DMEM Advance, L‑alanyl‑L‑glutamine is proven to grow any cell line properly.
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
