Roswell Park Memorial Institute 1640 Regular (RPMI -1640- Regular)

Roswell Park Memorial Institute 1640 Regular (RPMI -1640- Regular)

Price range: $21.00 through $37.00

Catalog Number: RPM-8010

Roswell Park Memorial Institute Medium 1640 (RPMI-1640) is commonly utilized for the cultivation of a variety of human cell types, including normal and cancerous leukocytes, as well as cell lines such as HeLa, Jurkat, MCF-7, PC12, PBMCs, astrocytes, carcinomas, bone
marrow cells, and hybridomas.

This medium is rich in phosphate, an essential nutrient for tumor cells, as they predominantly rely on glycolysis for energy production, especially in multicellular clusters. This metabolic adaptation is one of the key survival mechanisms employed by tumor cells.

 

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Description

RPMI-1640-Regular has proven to be effective for culturing a wide range of human cells, including both normal and cancerous leukocytes, as well as established lines such as HeLa, Jurkat, MCF-7, PC12, PBMCs, astrocytes, carcinomas, bone marrow cells, and hybridomas.

What sets RPMI-1640-Regular apart from other culture media, aside from its elevated pH level of 8, is its inclusion of biotin, vitamin B12, and para-aminobenzoic acid (PABA). Additionally, it features modified concentrations of amino acids and vitamins, with notably high levels of inositol and choline.

 

Parameter Specification
Appearance Red, clear liquid
pH  7.2 ± 0.1
Osmolality  275-360 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive  Sodium pyruvate
Indicator  Phenol red
Mycoplasma Detection Negative
Sterility Tested  Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

Additional information

Pack Size

1 × 500 mL, 6 × 500 mL, 1 × 1000 mL
Condition

DMEM – Standard Formulation, DMEM – Standard Formulation (ATCC-based), DMEM – Without L-Glutamine, DMEM – Without Phenol Red, DMEM – Without Sodium Bicarbonate, DMEM – With Sodium Pyruvate, DMEM – Without Glucose
DMEM – With Lipoic Acid, DMEM – Without L-Glutamine & Without Phenol Red, DMEM – Without L-Glutamine & Without Sodium Bicarbonate, DMEM – Without L-Glutamine, Phenol Red & Sodium Bicarbonate, DMEM – Low Glucose (1.5 g/L), DMEM – High Glucose (4.5 g/L), DMEM – Low Sodium Bicarbonate (1.5 g/L), DMEM – 15 mM HEPES (3.6 g/L), DMEM – 25 mM HEPES (5.9 g/L), DMEM – 15 mM HEPES + Low Sodium Bicarbonate (1.5 g/L), DMEM – 25 mM HEPES + Low Sodium Bicarbonate (1.5 g/L), DMEM – Without Phenol Red + 15 mM HEPES, DMEM – Without Phenol Red + 25 mM HEPES, DMEM – Without Phenol Red + Low Sodium Bicarbonate, DMEM – Without Phenol Red + 15 mM HEPES + Low Sodium Bicarbonate

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