Description
GMP Bioscience™ EMEM contains:
– Low glucose: 1.00 g/L
– Sodium bicarbonate: 1.5 g/L
– A high-bicarbonate variant is also available: 2.438 g/L sodium bicarbonate (Catalog #P3S032110)
– L-glutamine: 2 mM (292 mg/L)
– Sodium pyruvate: 1.0 mM (0.11 g/L)
– Enhanced concentrations of amino acids, vitamins, inositol, and choline to support metabolic demands
EMEM is formulated with components that reduce cell aggregation, making it well-suited for culturing tumor-derived and hybridoma cell lines.
GMP Bioscience scientists have applied this medium extensively over the years for a variety of primary and established cell lines, including:
– Human osteosarcoma (e.g., MG63)
– HEK293, HeLa, Jurkat, MCF-7, PC12
– PBMCs and various hybridomas
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
