Description
GMP Bioscience MEM Regular media in Hank’s buffer includes Hank’s buffer
Hank’s buffer, it contains:
- Potassium Chloride
- Potassium Phosphate, monobasic
- Sodium Bicarbonate
- Sodium Chloride
- Sodium Phosphate, dibasic
Additionally, MEM Regular in Hanks’ Buffer contains:
- Low level of glucose (1.00 g/ liter)
- Sodium bicarbonate (0.35 g/L). If, however, higher than 5% CO2 is used, a higher % of sodium bicarbonate is needed.
- 2 mM L-glutamine (292 mg/L)
MEM has been used for the cultivation of a wide variety of cells grown in monolayers attached or in suspension cell lines such as HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts as well as primary rat astrocytes. MEM Regular contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% fetal bovine serum (FBS).
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | N/A |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
