MEM-α-EBSS  Essential serves as an appropriate nutrient medium for mammalian cell culture and is also commonly used for the selection of transfected DHFR-negative cells. Researchers worldwide utilize MEM-α-EBSS for culturing primary osteoblasts as well as for isolating polymorphonuclear leukocytes (PMNs).

Background

MEM-α-EBSS Essential is well-established as an effective medium for mammalian cells and for selecting DHFR-negative transfected cells. It is extensively applied in primary osteoblast cultures and the isolation of PMNs from umbilical cord blood. The exclusion of calcium from this formulation supports the growth of cells in suspension culture systems.

Composition of MEM-α-EBSS MEM-α-EBSS includes:

– Non-essential amino acids
– Sodium pyruvate
– Lipoic acid
– Vitamin B12
– Biotin
– Ascorbic acid

Additionally, MEM-α-EBSS does not contain deoxyribonucleosides or ribonucleosides.

GMP Bioscience™ MEM-α-EBSS formulated in Earle’s Buffer contains the following salts:

1. Calcium chloride
2. Magnesium sulfate
3. Potassium chloride
4. Sodium bicarbonate
5. Sodium chloride
6. Monobasic sodium phosphate

Other notable components of MEM Alpha Modification in Earle’s Buffer include:

– Low glucose concentration (1.00 g/L)
– Sodium bicarbonate at 2.2 g/L (Note: for CO₂ concentrations above 5%, higher sodium bicarbonate levels are required)
– 2 mM L-glutamine (292 mg/L)

GMP Bioscience™ offers over 100 different MEM-α formulations to meet various research needs.

Applications

MEM-α-EBSS supports the culture of diverse suspension and adherent cell types, including keratinocytes, primary rat astrocytes, and human melanoma cells. Furthermore, this medium has been utilized in the isolation and culture of mesenchymal stem cells, adipose- derived stem cells, and osteoblasts.