Minimum Essential Medium Alpha-Modification in Hank’s Buffer (MEM-α-HBBS-Premium Plus)

MEM-α-HBBS-Premium Plus is formulated with L-Alanyl-L- Glutamine and additional components designed to support reduced-serum conditions. Notably, this medium requires only 5-7% FBS, ensuring more consistent experimental outcomes.

Overview

GMP Bioscience’s MEM-α-HBBS-Premium Plus is engineered to allow a substantial 30- 50% decrease in fetal bovine serum supplementation. The inclusion of defined, synthetic additives—such as recombinant insulin, albumin, and transferrin—enhances reproducibility and minimizes variability between serum lots. This formulation uses L-Alanyl-L-Glutamine as a stable glutamine source. By reducing dependence on FBS, this medium also helps lower the cost of cell culture protocols.

Composition

MEM-α-HBBS-Premium Plus contains:

– All 20 essential and non-essential amino acids, at higher concentrations than standard and premium formulations
– Specialized elements that greatly reduce serum dependency in cell culture systems.

Critical components:

– Human Transferrin (Holo): prevents improper iron storage and delivery in cell culture systems, which is a major contributor to oxidative stress and protein damage.

– Insulin Recombinant
– Trace of other elements that mimic the elements in fetal bovine serum.