DMEM/F-12 Ham 1:1 Mixture 1X, With L-glutamine, With HEPES

DMEM F-12 1:1 Nutrient Mixture Ham, 1X, with 365 mg/L L-glutamine and 15mM HEPES.

Catalog Number: DMF-9018
Lot Number: GMP24BS109

Product Details

DMEM F-12 is a 1:1 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM) and Ham’s F-12 Nutrient Mixture (Ham’s F-12). Widely used as a basal medium, DMEM F-12 combines DMEM’s high amino acid, glucose, and vitamin concentrations with growth factors and hormones found in Ham’s F-12. The result is an ideal medium capable of culturing a variety of cell lines.

DMF-9018 contains 365 mg/L L-glutamine and 15mM HEPES to compensate for the loss of buffering capacity incurred by eliminating serum. Important considerations when selecting a nutrient medium are: cell type, culture type (i.e., monolayer, suspension, clonal), and the degree of chemical definition necessary. It is important to review literature for recommendations concerning medium type, required supplementation, and physiological parameters required for a specific cell line.

Storage/Stability

The liquid medium should be stored at 2-8°C and stay away from direct sunlight. Deterioration of the liquid medium may be recognized by:

(1) Precipitate or particulate matter throughout the solution
(2) Cloudy appearance

(3) Color change
(4) pH change