DMEM/F-12 Ham 1:1 Mixture 1X, With L-glutamine, With HEPES, Without Sodium Bicarbonate

Dulbecco’s Modified Eagle’s Medium/F-12 Nutrient Mixture Ham (DMEM/F-12) 1:1 Mixtures, With 15mM HEPES, With 2.5mM L-glutamine, Without Sodium Bicarbonate

Catalog Number: DMF-9015
Lot Number: GMP24BS106

Product Details

F-12 Nutrient Mixture (Ham’s F-12) is a serum-free medium that relies on a combination of nutrient supplements, growth factors, and hormones to culture cells. During the past decade, researchers have reported successful culture of Leydig cells and Sertoli cells in serum-free medium that contained insulin, transferrin, epidermal growth factor (EGF), leutinizing hormone or follicle stimulating hormone, somatomedin and growth hormone. A 1:1 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM) and Ham’s F-12 Nutrient Mixture was found to be most satisfactory for studies of this type.

DMF-9015 contains 365 mg/L L-glutamine and 15mM HEPES to compensate for the loss of buffering capacity incurred by eliminating serum. DMF-9015 does not contain sodium bicarbonate.

Storage/Stability

The liquid medium should be stored at 2-8°C and stay away from direct sunlight. Deterioration of the liquid medium may be recognized by:

(1) Precipitate or particulate matter throughout the solution
(2) Cloudy appearance
(3) Color change
(4) pH change