F-12 Nutrient Mixture (HAM’S F-12) 1X, With L-alanyl-L- lutamine, Without HEPES

HAM’s F-12 is widely used to culture various mammalian and hybridoma cells. HMF-BBZ-XXX- 001 contains 217.23 mg/L L-alanyl-L-glutamine and is HEPES free.

Catalog Number: HMF-9026
Lot Number: GMP24BS117

Product Details

HAM’s F-12 was originally developed in the 1960’s for serum-free growth of Chinese hamster ovary (CHO) cells. Since then, it has been widely used, with and without supplemental serum, to culture various mammalian and hybridoma cells.

HMF-9026 contains 217.23 mg/L L-alanyl-L-glutamine and is HEPES free. Important considerations when selecting a nutrient medium include: cell type, culture type (i.e., monolayer, suspension, or clonal) and the degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium type, required supplementation and physiological parameters required for a specific cell line.

Storage/Stability

The liquid medium should be stored at 2-8°C away from direct sunlight. Deterioration of the liquid medium may be recognized by:

 Precipitate or particulate matter visible throughout the solution
 Cloudy appearance
 Color change
 pH change