F-12 Nutrient Mixture (HAM’S F-12) 1X, Without L-glutamine, Without HEPES
Catalog Number: HMF-9022
Lot Number: GMP24BS113
Product Details
HAM’s F-12 was originally developed in the 1960’s for serum-free growth of Chinese hamster ovary (CHO) cells. Since then, it has been widely used, with and without supplemental serum, to culture various mammalian and hybridoma cells.
HMF-9022 is L-glutamine and HEPES free. Important considerations when selecting a nutrient medium include: cell type, culture type (i.e., monolayer, suspension, or clonal) and the degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium type, required supplementation and physiological parameters required for a specific cell line.
Storage/Stability
The liquid medium should be stored at 2-8°C away from direct sunlight.
Deterioration of the liquid medium may be recognized by:
– Precipitate or particulate matter visible throughout the solution
– Cloudy appearance
– Color change
– pH change
| Media | With (+) | Without (-) | Cat No. | Size | Price |
|---|---|---|---|---|---|
| F-12 Nutrient Mixture (HAM’S F-12) 1X | – | L-glutamine
HEPES | HMF-9022 | 500 ml
1 lit 6 × 500 mL | $ 35
$ 60 $ 157 |
