MEM-α-N-EBSS is a nutritionally optimized medium designed to support mammalian cell culture and the selection of DHFR-negative transfected cells. This formulation includes ribonucleosides and deoxyribonucleosides, making it particularly well-suited for a variety of adherent and suspension cell types, depending on the experimental context.

Overview

MEM Alpha supplemented with nucleosides in Earle’s Balanced Salt Solution (EBSS) provides a refined environment for culturing a diverse range of mammalian cells. This medium facilitates the selection of DHFR-deficient cells, and its calcium-free formulation is particularly
advantageous for supporting suspension cell culture.

Composition

GMP Bioscience™ MEM-α-N-EBSS is a modified version of standard MEM that includes:

– Non-essential amino acids
– Sodium pyruvate
-Lipoic acid
– Vitamin B12
– Biotin
– Ascorbic acid

Additionally, it is fortified with Earle’s salts, which consist of:

– Calcium chloride
– Magnesium sulfate
– Potassium chloride
– Sodium bicarbonate
– Sodium chloride
– Sodium phosphate (monobasic)

The complete formulation also includes:

-Low glucose concentration (1.00 g/L)
– Sodium bicarbonate (2.2 g/L) — adjustable if higher CO₂ levels are used
– L-Alanyl-L-Glutamine (2 mM / 292 mg/L)

GMP Bioscience™ produces 121 variations of MEM-α-N-EBSS tailored to different laboratory needs.

Distinctive Additions

What differentiates MEM-α-N-EBSS from standard MEM-α is the inclusion of:

– Ribonucleosides
– Deoxyribonucleosides

These additions enhance nucleotide availability and support DNA/RNA synthesis during high metabolic demand.

Applications

MEM-α-N-EBSS is broadly used in culturing various primary and immortalized cell types,

including:

– Keratinocytes
– Primary rat astrocytes
-Human melanoma cells
– Mesenchymal stem cells
– Adipose-derived stem cells
– Osteoblasts
– Polymorphonuclear leukocytes (PMNs) from umbilical cord blood

Furthermore, it offers optimal conditions for the maintenance and propagation of primary osteoblast cultures.