Kaighn’s Modification of Ham’s F-12-Premium (Ham’s F-12K-Premium)

Kaighn’s Modification of Ham’s F-12-Premium (Ham’s F-12K-Premium)

 

Kaighn’s Modified Ham’s F-12-Premium is a widely utilized basal medium in the culture of mammalian cells. It is especially valued in both academic and industrial settings for its enriched buffering capacity and optimized nutrient profile.

Key Advantages over the Standard Ham’s F-12K

– Enhanced pH buffering capacity
–  Includes 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of traditional L-glutamine,

which:

–  Extends the stability and shelf life of the medium
–  Reduces ammonia accumulation, a toxic byproduct of glutamine degradation
–  Substantially improves cell viability and proliferation
–  Provides greater thermal stability, maintaining performance across varied storage and incubation conditions
– Facilitates improved growth of primary cell types

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Description

GMP Bioscience™ Ham’s F-12K-Premium is composed of:

–  5 mM sodium pyruvate
–  High glucose concentration: 4.5 g/L
–  Non-essential amino acids (NEAA)
–  3.7 g/L sodium bicarbonate

GMP Bioscience™ offers multiple variations of this medium depending on required sodium bicarbonate and HEPES buffering capacity Phenol red as a pH indicator

Please note that Ham’s F-12K-Premium is devoid of proteins, lipids, and growth-promoting factors. For optimal performance, supplementation with 10% fetal bovine serum (FBS) is recommended.

Mechanism:

While L-glutamine is an essential amino acid, it tends to break down over time, leading to the formation of harmful byproducts such as ammonia and pyrrolidine carboxylic acid. One strategy to reduce its degradation in culture media is by slowly supplementing it throughout the culture period. However, consistently monitoring and maintaining optimal levels can be labor-intensive and impractical.

A more efficient solution is to use L-alanyl-L-glutamine, which offers significantly greater stability in aqueous environments. Unlike free L-glutamine, it resists degradation and releases aminopeptidases at a controlled rate. This results in a sustained supply of L-glutamine and L- alanine, which are then utilized by cells for protein synthesis and energy metabolism via the TCA cycle.

Related products

HAM’S F-12K Essential Kaighn’s Modification of Ham’s F-12-K Medium Kaighn’s Modification of Ham’s F-12 Advance (Ham’s F-12K Advance)