MEM-Alpha with Nucleosides in Hanks’ Buffer Premium Plus

(MEM-α-N-HBSS-Premium Plus)

MEM-α-N-HBSS-Premium Plus is formulated with L-Alanyl-L-Glutamine along with specific components that support serum reduction. Typically, it requires only 5-7% FBS, offering greater
consistency in cell culture applications.

Background

MEM-α-N-HBSS-Premium Plus contains nucleosides and is enriched with ingredients that allow for a 30-50% decrease in the need for fetal bovine serum supplementation. A notable advantage is the inclusion of synthetic components such as recombinant insulin, albumin, and transferrin, which enhance batch-to-batch reproducibility. In this formulation, L-Alanyl-L- Glutamine is used as a more stable glutamine source. This also contributes to lowering overall costs associated with cell culture by reducing serum dependency.

Composition

MEM-α-N-HBSS-Premium Plus includes:

– All twenty essential and non-essential amino acids at elevated concentrations compared to standard and premium formulations

– Elements that substantially diminish serum requirements in culture
– Ribonucleosides
– Deoxyribonucleosides

Critical components:

– Human Transferrin (Holo): prevents improper iron storage and delivery in cell culture
systems, which is a major contributor to oxidative stress and protein damage.
– Insulin Recombinant
– Trace of other elements that mimic the elements in fetal bovine serum.