Description

Scientific Background:

GMP Bioscience Advance Kaighn’s Modification of Ham’s F-12 Medium (Ham’s F-12K Advance) is specifically developed to permit a 30-50% reduction in serum supplementation compared to conventional formulations. The inclusion of synthetic and recombinant components such as recombinant insulin, albumin, and transferrin enhances the medium’s consistency by minimizing the variability associated with serum lots. In this advanced formulation, L-Alanyl-L-Glutamine serves as a more stable glutamine source, offering extended shelf life and reduced ammonia accumulation. Additionally, the reduction in serum demand contributes to a significant decrease in cell culture costs.

GMP Bioscience Ham’s F-12K Advance includes:

–  All 20 Advance amino acids, provided at concentrations higher than those in the regular and premium versions

–  Specific components that substantially reduce the dependency on serum for optimal cell growth and maintenance

This formulation is particularly well-suited for researchers seeking greater control over experimental conditions and cost-effective media for serum-sensitive or large-scale cell culture workflows.

Critical components:

– Human Transferrin (Holo): prevents improper iron storage and delivery in cell culture systems, which is a major contributor to oxidative stress and protein damage.
– Insulin Recombinant
– Trace of other elements that mimic the elements in fetal bovine serum.