Description
GMP Bioscience IMDM Regular contains:
– Enhanced buffering capacity through elevated levels of sodium bicarbonate (3.02 g/L) and HEPES (5.9 g/L)
– High glucose concentration: 4.5 g/L
– Non-essential amino acids (NEAA)
– Sodium pyruvate: 110 mg/L (1.0 mM)
– Phenol red: 15 mg/L
– L-glutamine: 0.584 g/L
This formulation supports robust cell performance in both serum-supplemented and serum- reduced environments and is ideal for a variety of research and bioproduction applications requiring reliable, nutrient-rich culture conditions.
Mechanism:
While L-glutamine is an essential amino acid, it tends to break down over time, leading to the formation of harmful byproducts such as ammonia and pyrrolidine carboxylic acid. One strategy to reduce its degradation in culture media is by slowly supplementing it throughout the culture period. However, consistently monitoring and maintaining optimal levels can be labor-intensive and impractical.
A more efficient solution is to use L-alanyl-L-glutamine, which offers significantly greater stability in aqueous environments. Unlike free L-glutamine, it resists degradation and releases aminopeptidases at a controlled rate. This results in a sustained supply of L-glutamine and L- alanine, which are then utilized by cells for protein synthesis and energy metabolism via the TCA cycle.
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
