Description

Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible.

Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle.

In addition to our Advance formulation products, we manufacture GMP Bioscience GluaSup is a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.

Iscove’s Modified Dulbecco’s Medium Advance (IMDM-Advance) contains:

• 5 mM sodium pyruvate
• High glucose (4.5g/L)
• Non-Essential Amino Acids
• 3.7 g/L Sodium Bicarbonate; GMP Bioscience Manufactures several types of IMDM-Advance based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
• Phenol Red

This medium is ideal for applications requiring tight control over experimental conditions, including cell therapy, immunology, oncology, and biomanufacturing. Let me know if you need this content adapted into a catalog listing, brochure, or technical datasheet.

Critical components:

– Human Transferrin (Holo): prevents improper iron storage and delivery in cell culture systems, which is a major contributor to oxidative stress and protein damage.
– Insulin Recombinant
– Trace of other elements that mimic the elements in fetal bovine serum.