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MEM-α-N-EBSS Regular MEM Alpha with Nucleosides in Earle’s Buffer

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MEM Alpha with Nucleosides in Earle’s Buffer (MEM-α-N-EBSS) is a suitable nutrient for mammalian cell culture as well as selection for transfected DHFR-negative cells. This media contains nucleoside and, furthermore depending on cell types, laboratories use MEM-α-N for growing mammalian cells.

MEM Alpha with Nucleosides in Earle’s Buffer (MEM-α-N-EBSS) is a suitable nutrient for mammalian cell culture as well as selection for transfected DHFR-negative cells. This media contains nucleoside, and the formulation is available below. depending on cell types, laboratories use MEM-α-N for growing mammalian cell for a variety of suspension as well as adherent cells. Eliminating calcium in this media facilitates the growth of cells in suspension cultures.

 

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Description

Ingredients

MEM Alpha Modification with Nucleosides in Earle’s Buffer (MEM-α-N-EBSS) is a modification of MEM.

MEM-α-N-EBSS Regular contains:

  • Non-essential amino acids.
  • Sodium pyruvate.
  • Lipoic acid.
  • Vitamin B12.
  • Biotin and,
  • Ascorbic acid.

This media can also be used for keratinocytes, primary rat astrocytes, as well as human melanoma cells. MEM-α-N-EBSS has also been used for the isolation of mesenchymal stem cells, adipose-derived stem cells, and osteoblast. Additionally, GMP Bioscience MEM -α-N-EBSS facilitates the growth and maintenance of primary osteoblast cultures. furthermore, this media performs well for isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood.

 

Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

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MEM-α-EBSS Regular MEM Alpha Modification in Earle’s Buffer MEM-α-N-EBSS Advance MEM Alpha with Nucleosides in Earle’s Buffer