GMP Bioscience™ Eagle’s Minimal Essential Medium (EMEM) is a versatile basal medium suitable for both adherent and suspension cell lines.

Background

Originally developed by Harry Eagle, EMEM is a foundational cell culture medium composed of key salts, essential amino acids, glucose, and vitamins required for optimal cell proliferation and maintenance. Since its inception, the medium has undergone several modifications,
resulting in multiple enhanced formulations. EMEM remains widely utilized across academic and industrial laboratories due to its broad applicability to various mammalian cell types, including both adhesive and non-adhesive phenotypes.

Composition Details

GMP Bioscience™ EMEM contains:

– Low glucose: 1.00 g/L
– Sodium bicarbonate: 1.5 g/L
– A high-bicarbonate variant is also available: 2.438 g/L sodium bicarbonate (Catalog #P3S032110)

– L-glutamine: 2 mM (292 mg/L)
– Sodium pyruvate: 1.0 mM (0.11 g/L)
– Enhanced concentrations of amino acids, vitamins, inositol, and choline to support metabolic demands

Applications

EMEM is formulated with components that reduce cell aggregation, making it well-suited for culturing tumor-derived and hybridoma cell lines.

GMP Bioscience™ scientists have applied this medium extensively over the years for a variety of primary and established cell lines, including:

– Human osteosarcoma (e.g., MG63)
– HEK293, HeLa, Jurkat, MCF-7, PC12
– PBMCs and various hybridomas