Kaighn’s Modification of Ham’s F-12-K Medium (HAM’S F-12K Essential)

Kaighn’s Modification of Ham’s F-12-K Medium (HAM’S F-12K Essential)

Kaighn’s Modification of Ham’s F-12-Regular (Ham’s F-12K-Regular) is formulated with L- Alanyl-L-Glutamine, offering an extended shelf life and improved stability over traditional formulations. This version of the medium is specifically engineered to enhance both cell viability and growth performance. GMP Bioscience™ provides various customized formulations of Ham’s F- 2K-Regular based on the specific user requirements.

Scientific Background

Kaighn’s Modified Ham’s F-12-Premium is a widely utilized basal medium in the culture of mammalian cells. It is especially valued in both academic and industrial settings for its enriched buffering capacity and optimized nutrient profile.

Key Advantages over the Standard Ham’s F-12K

– Enhanced pH buffering capacity
–  Includes 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of traditional L-glutamine,

which:

–  Extends the stability and shelf life of the medium
–  Reduces ammonia accumulation, a toxic byproduct of glutamine degradation
–  Substantially improves cell viability and proliferation
–  Provides greater thermal stability, maintaining performance across varied storage and incubation conditions
– Facilitates improved growth of primary cell types

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Description

GMP Bioscience™ Ham’s F-12K-Regular is composed of:

–  5 mM sodium pyruvate
–  High glucose concentration: 4.5 g/L
–  Non-essential amino acids (NEAA)
–  3.7 g/L sodium bicarbonate

GMP Bioscience™ offers multiple variations of this medium depending on required sodium bicarbonate and HEPES buffering capacity Phenol red as a pH indicator

Please note that Ham’s F-12K-Premium is devoid of proteins, lipids, and growth-promoting factors. For optimal performance, supplementation with 10% fetal bovine serum (FBS) is recommended.

Mechanism:

While L-glutamine is an essential amino acid, it tends to break down over time, leading to the formation of harmful byproducts such as ammonia and pyrrolidine carboxylic acid. One strategy to reduce its degradation in culture media is by slowly supplementing it throughout the culture period. However, consistently monitoring and maintaining optimal levels can be labor-intensive and impractical.

A more efficient solution is to use L-alanyl-L-glutamine, which offers significantly greater stability in aqueous environments. Unlike free L-glutamine, it resists degradation and releases aminopeptidases at a controlled rate. This results in a sustained supply of L-glutamine and L- alanine, which are then utilized by cells for protein synthesis and energy metabolism via the TCA cycle.

 

Parameter Specification
Appearance Red, clear liquid
pH  7.2 ± 0.1
Osmolality  275-360 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive  Sodium pyruvate
Indicator  Phenol red
Mycoplasma Detection Negative
Sterility Tested  Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

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